13 Nov 2023

Job Information

Organisation/Company

Université de Limoges

Department

DRH-RECHERCHE

Research Field

Biological sciences » Biology

Researcher Profile

Established Researcher (R3)

Country

France

Application Deadline

11 Jan 2024 - 23:59 (Europe/Paris)

Type of Contract

Temporary

Job Status

Full-time

Offer Starting Date

1 Apr 2024

Is the job funded through the EU Research Framework Programme?

Not funded by an EU programme

Is the Job related to staff position within a Research Infrastructure?

No

Offer Description

Among women, breast cancer (BC) is the most common cause of cancer, and the second leading cause of cancer death. The molecular classification of BC distinguishes luminal A and B expressing estrogen (ER) and/or progesterone receptors (PR), basal/triple negative breast cancer (TNBC), and human epidermal growth factor receptor 2 (HER2)-like tumors. This molecular taxonomy is clinically relevant with basal/TNBC patients presenting the poorest clinical outcome with no targeted therapies available when compared to other molecular subtypes. CD95 belongs to the tumor necrosis factor (TNF) receptor family and has mainly been viewed as a death receptor (Peter et al., 2015). However, recent data highlighted that this receptor can also induce non-apoptotic signaling pathways involved in physiological processes (Desbarats et al., 2003; Desbarats and Newell, 2000) or in the progression of auto-immune (O' Reilly et al., 2009; Poissonnier et al., 2016; Tauzin et al., 2011) and cancer disorders (Barnhart et al., 2004; Kleber et al., 2008). TNBCs maintain a very high amount of surface CD95 when compared to other breast cancers, and we recently found that CD95 loss in TNBC cells reprograms the immune landscape, by triggering a pro-inflammatory response unleashing the anti-tumor activity of natural killer (NK) cells. The CD95-driven molecular mechanism responsible for the recruitment and activation of NK cells in TNBC remains to be elucidated. This program proposes to address the molecular mechanism leading to NF-kB activation in CD95 deficient TNBC cells and hierarchize this signal using different molecular and biochemical assays.

Requirements

Research Field

Biological sciences » Biology

Education Level

PhD or equivalent

Skills/Qualifications

Knowledge in cell signaling -Skills in molecular biology -Experience in biochemistry (IP, western blot) and cell biology (flow cytometry)

Specific Requirements

- Molecular biology (cell cloning, Crispr/Cas9, protein expression in mammalian cells, protein expression in bacteria, protein purification) - Cell culture (generation of stable clones, cell sorting, flow cytometry) - Biochemistry (immunoprecipitation, western blot, gel filtration) -Animal experimentation could be a plus

Languages

FRENCH

Level

Basic

Languages

ENGLISH

Level

Excellent

Research Field

Biological sciences » Biology

Additional Information

Benefits

12 months contract (possibility to be renewed 12 monhs). Gross salary between 2600 and 3000 euros/months upon experience. Starting first semester 2022. Applicants are invited to send a CV (with list of publications, research experiences and referees)

Eligibility criteria

According to French legislation, the applicant must have received a PhD degree no later than three years before the beginning of the contract

Selection process

CV, cover letter and copy of PhD degree to be sent by email mentioning reference of job offer at : [email protected]

Website for additional job details

https: // www. unilim.fr/recrutement-post-doctorant/

Work Location(s)

Number of offers available

1

Company/Institute

Ω-Health

Country

France

City

LIMOGES

Postal Code

87025

Street

2, rue du docteur Marcland

Geofield

Where to apply

Website

https: // www. unilim.fr/recrutement-post-doctorant/

Contact

City

Limoges

Website

https: // www. unilim.fr/

Street

33 Rue François Mitterrand

Postal Code

87000

STATUS: EXPIRED